Let’s break through that biofilm

Presented by Charlie Pye, BSc, DVM, DVSc, Dipl. ACVD

Continuing education questionnaire

CE credit: 1

AssignedWebcast

Name

First name *

Last name *

License number *

Province where you practise *

Email *

Questions

1. What is a biofilm? *

A community of bacteria that moves around from surface to surface

A community of bacteria that are attached to a surface and encased in an extracellular matrix

A documentary about different bacterial species

Viruses that attack and penetrate bacterial cells

2. Which of the following surfaces have bacteria been documented to form biofilm on? *

Teeth

Catheters

Surgical implants

All of the above

3. On cytology, if a biofilm has formed, you may see the following: *

Bacteria surrounded by “veil-like” background staining

Bacteria and neutrophils that are too numerous to count

Bacteria will not be noted as the biofilm is surrounding them

Sheets of keratinocytes with occasional bacteria

4. Bacteria within a biofilm can be more resistant to antibiotics due to: *

Excess of nutrients which leads to rapid cellular growth

Higher oxygen levels within the biofilm

Overproduction of efflux pumps in the cell wall

Bacteria in biofilms are not more resistant to antibiotics

5. Which of the following treatments has not been shown to be effective at disrupting/preventing biofilm formation? *

Bacteriophages

Microsilver

Saline

TrizEDTA

6. True or False. Bacteria begin to produce extrapolymeric substances (EPS) when they irreversibly attach to a surface. *

True

False

7. True or False. Pseudomonas aeruginosa does not form biofilms. *

True

False

8. Ophytrium has which of the following properties? *

Limits biofilm formation

Limits adhesion of bacteria

Strengthens the skin barrier

All of the above

9. True or False. A biofilm forming in the external ear canal can act as a perpetuating factor in otitis development. *

True

False

10. True or False. Bacteria within a biofilm are more susceptible when the biofilm is disrupted. *

True

False